HIGH-FREQUENCY TRANSFER OF LINEAR DNA CONTAINING 5'-COVALENTLY LINKED TERMINAL PROTEINS - ELECTROPORATION OF BACTERIOPHAGE-PRD1 GENOME INTO ESCHERICHIA-COLI

被引：34

作者：

LYRA, C ^{[1
]}

SAVILAHTI, H ^{[1
]}

BAMFORD, DH ^{[1
]}

机构：

[1] UNIV HELSINKI,DEPT GENET,ARKADIANKATU 7,SF-00100 HELSINKI 10,FINLAND

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 228卷 / 1-2期

关键词：

LINEAR DNA; ELECTROPORATION; TRANSFECTION; DNA TERMINAL PROTEIN; BACTERIOPHAGE-PRD1;

D O I：

10.1007/BF00282449

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using electroporation with the phage PRD1 genome, we set up a high-frequency DNA transfer system for a linear dsDNA molecule with 5'-covalently linked terminal proteins. The transfer was saturated when more than 100 ng of PRD1 genome was used. Electroporation efficiency was about four orders of magnitude higher than that obtained with transfection. Removal of the terminal protein abolished plaque formation, which could not be rescued by supplying the terminal protein or phage DNA polymerase or both in trans.

引用

页码：65 / 69

页数：5

共 38 条

[1] IDENTIFICATION OF A PROTEIN-BOUND TO THE TERMINI OF BACTERIOPHAGE-PRD1 DNA
BAMFORD, D
MCGRAW, T
MACKENZIE, G
MINDICH, L
[J]. JOURNAL OF VIROLOGY, 1983, 47 (02) : 311 - 316
[2] STRUCTURE OF THE LIPID-CONTAINING BACTERIOPHAGE-PRD1 - DISRUPTION OF WILD-TYPE AND NONSENSE MUTANT PHAGE PARTICLES WITH GUANIDINE-HYDROCHLORIDE
BAMFORD, D
MINDICH, L
[J]. JOURNAL OF VIROLOGY, 1982, 44 (03) : 1031 - 1038
[3] COMPARISON OF THE LIPID-CONTAINING BACTERIOPHAGE-PRD1, BACTERIOPHAGE-PR3, BACTERIOPHAGE-PR4, BACTERIOPHAGE-PR5 AND BACTERIOPHAGE-L17
BAMFORD, DH
ROUHIAINEN, L
TAKKINEN, K
SODERLUND, H
[J]. JOURNAL OF GENERAL VIROLOGY, 1981, 57 (DEC) : 365 - 373
[4] CHARACTERIZATION OF THE DNA-PROTEIN COMPLEX AT THE TERMINI OF THE BACTERIOPHAGE PRD1 GENOME
BAMFORD, DH
MINDICH, L
[J]. JOURNAL OF VIROLOGY, 1984, 50 (02) : 309 - 315
[5] CAPSOMER PROTEINS OF BACTERIOPHAGE PRD1, A BACTERIAL-VIRUS WITH A MEMBRANE
BAMFORD, JKH
BAMFORD, DH
[J]. VIROLOGY, 1990, 177 (02) : 445 - 451
[6] LARGE-SCALE PURIFICATION OF MEMBRANE-CONTAINING BACTERIOPHAGE PRD1 AND ITS SUBVIRAL PARTICLES
BAMFORD, JKH
BAMFORD, DH
[J]. VIROLOGY, 1991, 181 (01) : 348 - 352
[7] BAMFORD JKH, 1991, IN PRESS VIROLOGY, V183
[8] DNA-SYNTHESIS IN PURIFIED MAIZE MITOCHONDRIA
BEDINGER, P
WALBOT, V
[J]. CURRENT GENETICS, 1986, 10 (08) : 631 - 637
[9] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[10] STRUCTURE AND ASSEMBLY OF BACTERIOPHAGE-PRD1, AN ESCHERICHIA-COLI VIRUS WITH A MEMBRANE
CALDENTEY, J
BAMFORD, JKH
BAMFORD, DH
[J]. JOURNAL OF STRUCTURAL BIOLOGY, 1990, 104 (1-3) : 44 - 51

← 1 2 3 4 →