OPTIMIZATION OF MICROSATELLITE ANALYSIS FOR GENETIC-MAPPING

被引：40

作者：

HUGHES, AE

机构：

[1] Department of Medical Genetics, The Queen's University of Belfast, Belfast City Hospital

来源：

GENOMICS | 1993年 / 15卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/geno.1993.1083

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method for typing microsatellite polymorphisms is described. It involves amplification using the polymerase chain reaction with one primer 5' end-labeled with 32P. Alleles are separated by denaturing gel electrophoresis and detected by autoradiography. Standardized conditions allow accurate typing of almost all microsatellite polymorphisms, and results are usually obtained within 24 h. © 1993 Academic Press, Inc.

引用

页码：433 / 434

页数：2