High-performance ion exchange chromatography of pectins

被引：31

作者：

Schols, H. A. ^{[1
]}

Reitsma, J. C. E. ^{[1
]}

Voragen, A. G. J. ^{[1
]}

Pilnik, W. ^{[1
]}

机构：

[1] Univ Agr, Dept Food Sci, NL-6703 HD Wageningen, Netherlands

来源：

FOOD HYDROCOLLOIDS | 1989年 / 3卷 / 02期

关键词：

D O I：

10.1016/S0268-005X(89)80021-9

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A method for the rapid analysis of the molecular distribution of methoxyl groups along pectin molecules on a non-porous anion exchange resin using HPLC has been developed. The results obtained were similar to results obtained with conventional ion exchange chromatography over DEAE columns. However, the analysis time was 30 min instead of 1-2 days. With this HPLC method the differences in the pattern of enzymic demethylation of highly esterified pectin by fungal and citrus pectin esterase could be conveniently demonstrated.

引用

页码：115 / 121

页数：7

共 17 条

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