NUCLEOCYTOPLASMIC INTERACTIONS IN EXPERIMENTAL BINUCLEATES FORMED FROM NORMAL AND TRANSFORMED COMPONENTS

被引：18

作者：

MUGGLETONHARRIS, AL

PALUMBO, M

机构：

来源：

SOMATIC CELL GENETICS | 1979年 / 5卷 / 03期

关键词：

D O I：

10.1007/BF01538850

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for nuclear transfer and fusion of individual viable nuclei into cells is described. Experimental binucleates were formed by fusing nuclei from normal [human] fibroblasts, SV40 virus-transformed fibroblasts, and HeLa [human cervical] carcinoma cells into individual diploid fibroblasts which have undergone their total life span (population doublings) and have been maintained in culture for 10-12 wk (late phase III) prior to the nuclear transfer. Many of the cells receiving additional nuclei replicate and form clones of cells with a limited life span, and the morphology of the cells remains fibroblast-like.

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页码：397 / 407

页数：11

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