TRANSFORMATION OF MYCOBACTERIUM-AURUM AND MYCOBACTERIUM-SMEGMATIS WITH THE BROAD HOST-RANGE GRAM-NEGATIVE COSMID VECTOR PJRD215

被引：26

作者：

HERMANS, J

MARTIN, C

HUIJBERTS, GNM

GOOSEN, T

DEBONT, JAM

机构：

[1] INST PASTEUR,UNITE GENIE MICROBIOL,F-75724 PARIS 15,FRANCE

[2] AGR UNIV WAGENINGEN,DEPT GENET,WAGENINGEN,NETHERLANDS

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb00803.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transformation of Mycobacterium aurum and Mycobacterium smegmatis with the Gram-negative RSF1010-derived cosmid pJRD215 is described. The plasmid is stably maintained in both species and the antibiotic resistance determinants for kanamycin and streptomycin are expressed. Southern blot analysis shows that rearrangements take place both in M. aurum and in M. smegmatis. The use of pJRD215 in mycobacterial cloning systems is discussed.

引用

页码：1561 / 1566

页数：6

共 24 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] THE EXPRESSION OF STREPTOMYCES AND ESCHERICHIA-COLI DRUG-RESISTANCE DETERMINANTS CLONED INTO THE STREPTOMYCES PHAGE PHI-C31 [J].