APPLICATION OF SPIN-ECHO NUCLEAR MAGNETIC-RESONANCE TO WHOLE-CELL SYSTEMS - MEMBRANE-TRANSPORT

被引：169

作者：

BRINDLE, KM

BROWN, FF

CAMPBELL, ID

GRATHWOHL, C

KUCHEL, PW

机构：

来源：

BIOCHEMICAL JOURNAL | 1979年 / 180卷 / 01期

关键词：

D O I：

10.1042/bj1800037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for studying membrane transport is presented. High resolution n.m.r. is used to measure the distribution of small molecules between the intracellular and extracellular compartments. The method uses spin-echo techniques and relies on a difference in the magnetic susceptibility of the media inside and outside of cells. It also provides simultaneous information on the metabolic status of the cell. The method is illustrated by a study of alanine and lactate transport in the human erythrocyte.

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页码：37 / 44

页数：8

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