SYNAPTOCANALIN-I, A PROTEIN ASSOCIATED WITH BRAIN OMEGA-CONOTOXIN-SENSITIVE CALCIUM CHANNELS

被引：33

作者：

MORITA, T

MORI, H

SAKIMURA, K

MISHINA, M

SEKINE, Y

TSUGITA, A

ODANI, S

HORIKAWA, HPM

SAISU, H

ABE, T

机构：

[1] NIIGATA UNIV,FAC SCI,DEPT BIOL,NIIGATA 951,JAPAN

[2] NIIGATA UNIV,BRAIN RES INST,DEPT NEUROPHARMACOL,NIIGATA 951,JAPAN

[3] SCI UNIV TOKYO,BIOSCI RES INST,NODA,CHIBA 278,JAPAN

来源：

BIOMEDICAL RESEARCH-TOKYO | 1992年 / 13卷 / 05期

关键词：

D O I：

10.2220/biomedres.13.357

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We have previously identified two membrane proteins (36 kDa and 28 kDa) associated with omega-conotoxin GVIA-sensitive (N-type) calcium channels in the brain. Partial amino acid sequences and immunoblot analysis after sodium dodecyl sulfate-electrophoresis in urea indicated the presence of several isoforms (synaptocanalins) of the 36 kDa protein in the brain. The amino acid sequence of one form of the 36 kDa protein (named synaptocanalin I) deduced from its cDNA indicates that it is a novel protein with significant homology to epimorphin, a protein involved in epithelial morphogenesis. A stretch (24 residues) of very hydrophobic amino acids at its carboxy terminus is proposed as its only membrane-spanning domain.

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页码：357 / 364

页数：8

相关论文

共 24 条

[1] SYNTAXIN - A SYNAPTIC PROTEIN IMPLICATED IN DOCKING OF SYNAPTIC VESICLES AT PRESYNAPTIC ACTIVE ZONES [J].

BENNETT, MK ;

CALAKOS, N ;

SCHELLER, RH .

SCIENCE, 1992, 257 (5067) :255-259

[2] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[3] REGULATION OF CALCIUM-CHANNEL ACTIVITY BY GTP BINDING-PROTEINS AND 2ND MESSENGERS [J].

DOLPHIN, AC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1091 (01) :68-80

[4] MOLECULAR-CLONING OF THE ALPHA-1 SUBUNIT OF AN OMEGA-CONOTOXIN-SENSITIVE CALCIUM-CHANNEL [J].

DUBEL, SJ ;

STARR, TVB ;

HELL, J ;

AHLIJANIAN, MK ;

ENYEART, JJ ;

CATTERALL, WA ;

SNUTCH, TP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :5058-5062

[5]

GRAY WR, 1988, ANNU REV BIOCHEM, V57, P665, DOI 10.1146/annurev.bi.57.070188.003313

[6] MOLECULAR-WEIGHT OF MAJOR GLYCOPROTEIN FROM HUMAN ERYTHROCYTE-MEMBRANE [J].

GREFRATH, SP ;

REYNOLDS, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (10) :3913-3916

[7] ELUTION OF PROTEINS FROM SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS, REMOVAL OF SODIUM DODECYL-SULFATE, AND RENATURATION OF ENZYMATIC-ACTIVITY - RESULTS WITH SIGMA SUBUNIT OF ESCHERICHIA-COLI RNA-POLYMERASE, WHEAT-GERM DNA TOPOISOMERASE, AND OTHER ENZYMES [J].

HAGER, DA ;

BURGESS, RR .

ANALYTICAL BIOCHEMISTRY, 1980, 109 (01) :76-86

[8] THE HIGH-AFFINITY RECEPTOR FOR OMEGA-CONOTOXIN REPRESENTS CALCIUM CHANNELS DIFFERENT FROM THOSE SENSITIVE TO DIHYDROPYRIDINES IN MAMMALIAN BRAIN [J].

HAYAKAWA, N ;

MORITA, T ;

YAMAGUCHI, T ;

MITSUI, H ;

MORI, KJ ;

SAISU, H ;

ABE, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 173 (02) :483-490

[9] EPIMORPHIN - A MESENCHYMAL PROTEIN ESSENTIAL FOR EPITHELIAL MORPHOGENESIS [J].

HIRAI, Y ;

TAKEBE, K ;

TAKASHINA, M ;

KOBAYASHI, S ;

TAKEICHI, M .

CELL, 1992, 69 (03) :471-481

[10]

INOUE A, 1992, J BIOL CHEM, V267, P10613