COMPARATIVE-STUDY ON FLEXIBACTER-MARITIMUS STRAINS ISOLATED FROM FARMED SEA BASS (DICENTRARCHUS-LABRAX) IN FRANCE

被引：54

作者：

BERNARDET, JF

KEROUALT, B

MICHEL, C

机构：

来源：

FISH PATHOLOGY | 1994年 / 29卷 / 02期

关键词：

D O I：

10.3147/jsfp.29.105

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Following severe outbreaks of a disease with dermal necrosis, several bacterial strains were isolated from sea bass (Dicentrarchus labrax) reared on French coasts. A phenotypic study revealed that the isolates closely resembled Flexibacter maritimus. Nine selected strains were compared to 8 F. maritimus reference strains from other geographical origins and to the type strain NCIMB 2154T. DNA base composition of the strains tested was in the G + C range of 30.6 to 32mol%. A study on the DNA relatedness among the strains by the S1 nuclease DNA-DNA hybridization method showed that the 12 strains tested formed a tight genomic species with DNA homologies above 74%. The profiles obtained from API ZYM galleries, polyacrylamide gel electrophoresis of whole-cell proteins, and fatty acids analysis revealed that F. maritimus is a very homogeneous bacterial species. This is the first identification of this fish pathogen in France and its first isolation from sea bass.

引用

页码：105 / 111

页数：7

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