REGULATION OF BOVINE ADRENAL-CELL CORTICOTROPIN RECEPTOR MESSENGER-RNA LEVELS BY CORTICOTROPIN (ACTH) AND ANGIOTENSIN-II (A-II)

被引：49

作者：

PENHOAT, A ^{[1
]}

JAILLARD, C ^{[1
]}

SAEZ, JM ^{[1
]}

机构：

[1] HOP DEBROUSSE,INSERM,U307,F-69322 LYON,FRANCE

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1994年 / 103卷 / 1-2期

关键词：

ADRENOCORTICOTROPIN; ANGIOTENSIN-II; ADRENOCORTICOTROPIN RECEPTOR; BOVINE ADRENOCORTICAL CELL;

D O I：

10.1016/0303-7207(94)90088-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Bovine adrenal fasciculata-reticularis cells (BAC) expressed at least four ACTH receptor (ACTH-R) mRNA transcripts, one major of 3.6 kb and three minor of 4.2, 1.8 and 1.3 kb. ACTH and A-II increased ACTH-R mRNA levels in a time- and dose-dependent manner. At maximal concentrations, ACTH caused a 2.7-fold increase in the level of the major transcript of 3.6 kb with an ED(50) = 10(-11) M and A-II produced a 2.4-fold increase with an ED(50) = 5 X 10(-8) M. Under our experimental conditions, the stimulatory effects of both hormones appeared to be due to post-transcriptional changes rather than to transcriptional regulation since the hormonal effects were also observed in actinomycin-treated cells. The results indicate that regulation of ACTH-R mRNA levels may be one mechanism by which ACTH and A-II regulate adrenocortical functions.

引用

页码：R7 / R10

页数：4