PARTITIONING BEHAVIOR IN AQUEOUS 2-PHASE SYSTEMS AND FRACTIONATION BY COUNTERCURRENT DISTRIBUTION OF CHICK-EMBRYO ERYTHROCYTES WITH NUMERICAL RESOLUTION OF DISTRIBUTION CURVES

被引：2

作者：

FERRER, E

MARTIN, M

ALONSO, R

LUQUE, J

机构：

[1] UNIV ALCALA HENARES, DEPT BIOQUIM & BIOL MOLEC, ALCALA HENARES, SPAIN

[2] UNIV COMPLUTENSE MADRID, FAC VET, DEPT BIOQUIM, E-28040 MADRID, SPAIN

[3] UNIV POLITECN MADRID, ESCUELA TECN SUPERIOR INGN AGRON, UNIDAD ESTADIST, E-28040 MADRID, SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY | 1988年 / 445卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)84545-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The partition of chick-embryo and young-chick erythrocytes in dextran-poly-(ethylene glycol) two-phase systems depends on the interfacial tension and electrical potential differences between the phases. Counter-current distribution with charged 5% dextran-poly(ethylene glycol) systems has proved to be an adequate method for the separation of primitive and definitive erythrocytes present in chick embryos when a phase settling time of 20 min is used. The computer-aided numerical resolution of experimental curves has shown the existence of subpopulations which could not have been detected by using conventional methods.

引用

页码：327 / 336

页数：10

共 26 条

[1]

Albertsson P.A., 1986, PARTITION CELL PARTI

[2]

BARTLETT GR, 1980, AM ZOOL, V20, P103

[3]

Beaupain D, 1981, HEMOGLOBINS DEV DIFF, P161

[4] NUMERICAL RESOLUTION OF CCD-CURVES [J].

BLOMQUIST, G ;

WOLD, S .

ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY, 1974, B 28 (01) :56-60

[5]

BROWN JL, 1974, J BIOL CHEM, V249, P3960

[6]

CHAPMAN BS, 1981, HEMOGLOBINS DEV DIFF, P193

[7]

DIETERLENLIEVRE F, 1987, NATO ASI SERIES H, V8, P127

[8] SEPARATION BY COUNTERCURRENT DISTRIBUTION OF YOUNG-CHICK ERYTHROCYTE POPULATIONS AND NUMERICAL RESOLUTION OF DISTRIBUTION CURVES [J].

FERRER, E ;

MARTIN, M ;

ALONSO, R ;

LUQUE, J .

JOURNAL OF CHROMATOGRAPHY, 1987, 411 :167-176

[9]

FERRER E, UNPUB

[10] CELL SEPARATIONS AND SUBFRACTIONATIONS BY COUNTERCURRENT DISTRIBUTION IN 2-POLYMER AQUEOUS PHASE SYSTEMS DEPEND ON NON-EQUILIBRIUM CONDITIONS [J].

FISHER, D ;

WALTER, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 801 (01) :106-110

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