PARTITIONING BEHAVIOR IN AQUEOUS 2-PHASE SYSTEMS AND FRACTIONATION BY COUNTERCURRENT DISTRIBUTION OF CHICK-EMBRYO ERYTHROCYTES WITH NUMERICAL RESOLUTION OF DISTRIBUTION CURVES

被引：2

作者：

FERRER, E

MARTIN, M

ALONSO, R

LUQUE, J

机构：

[1] UNIV ALCALA HENARES, DEPT BIOQUIM & BIOL MOLEC, ALCALA HENARES, SPAIN

[2] UNIV COMPLUTENSE MADRID, FAC VET, DEPT BIOQUIM, E-28040 MADRID, SPAIN

[3] UNIV POLITECN MADRID, ESCUELA TECN SUPERIOR INGN AGRON, UNIDAD ESTADIST, E-28040 MADRID, SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY | 1988年 / 445卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)84545-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The partition of chick-embryo and young-chick erythrocytes in dextran-poly-(ethylene glycol) two-phase systems depends on the interfacial tension and electrical potential differences between the phases. Counter-current distribution with charged 5% dextran-poly(ethylene glycol) systems has proved to be an adequate method for the separation of primitive and definitive erythrocytes present in chick embryos when a phase settling time of 20 min is used. The computer-aided numerical resolution of experimental curves has shown the existence of subpopulations which could not have been detected by using conventional methods.

引用

页码：327 / 336

页数：10

共 26 条

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FISHER D, 1981, BIOCHEM J, V196, P1

[12] PARTITIONING BEHAVIOR OF RAT BONE-MARROW CELLS IN AQUEOUS 2-PHASE SYSTEMS - DEPENDENCE OF CELL PARTITION ON THE INTERFACIAL-TENSION AND ELECTRICAL POTENTIAL DIFFERENCE BETWEEN THE PHASES [J].