NADPH-INDUCED MICROSOMAL CHEMI-LUMINESCENCE WITHOUT ACCOMPANYING LIPID PEROXIDATION AS MEASURED BY THE THIOBARBITURIC ACID ASSAY

被引:7
作者
GUTHANS, SL [1 ]
BARICOS, WH [1 ]
STEELE, RH [1 ]
机构
[1] TULANE UNIV,MED CTR,DEPT BIOCHEM,NEW ORLEANS,LA 70112
关键词
(Microsome); Chemiluminescence; NADPH; Peroxidation; Redox chain; Thiobarbituric acid assay;
D O I
10.1016/0304-4165(79)90409-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pyrophosphate added to rat liver microsomes blocks both NADPH-induced chemiluminescence and lipid peroxidation, whether NADPH is added in bulk or generated with an NADPH-regeneration system. Addition of KCN to these systems released the pyrophosphate suppression of NADPH-induced chemiluminescence without restoring lipid peroxidation. A hexose or hexose derivative is required for the display of KCN-released chemiluminescence with galactose 6-phosphate and glucose 6-phosphate being most effective. The integral KCN-released chemiluminescence for pyrophosphate inhibited systems is proportional to microsomal protein, NADPH, and glucose-6-phosphate dehydrogenase concentrations. Pyrophosphate also blocks ascorbic acid induced chemiluminescence. Additionally ascorbic acid inhibits the KCN release of NADPH-induced chemiluminescence. This work establishes a point a divergence in the microsomal redox chain between NADPH-induced chemiluminescence and lipid peroxidation as measured by the thiobarbituric acid assay. © 1979.
引用
收藏
页码:112 / 122
页数:11
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