NADPH-INDUCED MICROSOMAL CHEMI-LUMINESCENCE WITHOUT ACCOMPANYING LIPID PEROXIDATION AS MEASURED BY THE THIOBARBITURIC ACID ASSAY

被引：7

作者：

GUTHANS, SL ^{[1
]}

BARICOS, WH ^{[1
]}

STEELE, RH ^{[1
]}

机构：

[1] TULANE UNIV,MED CTR,DEPT BIOCHEM,NEW ORLEANS,LA 70112

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 586卷 / 01期

关键词：

(Microsome); Chemiluminescence; NADPH; Peroxidation; Redox chain; Thiobarbituric acid assay;

D O I：

10.1016/0304-4165(79)90409-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pyrophosphate added to rat liver microsomes blocks both NADPH-induced chemiluminescence and lipid peroxidation, whether NADPH is added in bulk or generated with an NADPH-regeneration system. Addition of KCN to these systems released the pyrophosphate suppression of NADPH-induced chemiluminescence without restoring lipid peroxidation. A hexose or hexose derivative is required for the display of KCN-released chemiluminescence with galactose 6-phosphate and glucose 6-phosphate being most effective. The integral KCN-released chemiluminescence for pyrophosphate inhibited systems is proportional to microsomal protein, NADPH, and glucose-6-phosphate dehydrogenase concentrations. Pyrophosphate also blocks ascorbic acid induced chemiluminescence. Additionally ascorbic acid inhibits the KCN release of NADPH-induced chemiluminescence. This work establishes a point a divergence in the microsomal redox chain between NADPH-induced chemiluminescence and lipid peroxidation as measured by the thiobarbituric acid assay. © 1979.

引用

页码：112 / 122

页数：11

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