PURIFICATION AND CHARACTERIZATION OF ADP-GLUCOSE PYROPHOSPHORYLASE-A FROM DEVELOPING MAIZE SEEDS

被引：14

作者：

FUCHS, RL ^{[1
]}

SMITH, JD ^{[1
]}

机构：

[1] TEXAS A&M UNIV, DEPT PLANT SCI, GENET SECT, COLLEGE STN, TX 77843 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 566卷 / 01期

关键词：

(Maize; Purification; Properties); ADPglucose; Development; Pyrophosphorylase A;

D O I：

10.1016/0005-2744(79)90246-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ADPglucose pyrophosphorylase A (ATP: α-d-glucose-1-phosphate adenylyl-transferase, EC 2.7.7.27) from developing maize (Zea mays) endosperm was purified 129 fold to apparent homogeneity. The molecular weight estimation by gel filtration and by polyacrylamide gel electrophoresis was 375 000 and 400 000, respectively. The preparation gave a single protein band after SDS-polyacrylamide gel electrophoresis suggesting a monomer mol. wt. of 96 000. It was concluded that ADPglucose pyrophosphorylase A in maize endosperm is a tetramer of four similar molecular weight subunits. Values for the Km for glucose 1-phosphate and ATP were 3.8 · 10-5 and 1.8 · 10-4 M, respectively (using the homogeneous preparation). © 1979.

引用

页码：40 / 48

页数：9

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