WHAT SPECIES IS RESPONSIBLE FOR STRAND SCISSION IN THE REACTION OF [FE(II)EDTA](2-) AND H2O2 WITH DNA

被引：142

作者：

POGOZELSKI, WK ^{[1
]}

MCNEESE, TJ ^{[1
]}

TULLIUS, TD ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,DEPT CHEM,BALTIMORE,MD 21218

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1995年 / 117卷 / 24期

关键词：

D O I：

10.1021/ja00129a002

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The reaction of ferrous iron with hydrogen peroxide (the Fenton reaction) has been widely used as a means of inducing strand cleavage in DNA, generally for the purpose of ''footprinting'' protein-DNA complexes or studying DNA structure. The identity of the DNA-oxidizing species produced in the reaction, however, has been an issue of debate. By comparing gamma-radiolysis-induced DNA cleavage patterns in a variety of buffers with those generated by the reaction of [Fe(EDTA)](2-) + H2O2 in the presence of ascorbate, we report that under the conditions used for a typical footprinting experiment, results are consistent with production of the hydroxyl radical as the oxidant responsible for DNA strand scission. We also cite experiments that argue against the participation of a high-valent iron-ore complex in DNA cutting.

引用

页码：6428 / 6433

页数：6

共 57 条

[21]

Halliwell B., 1985, FREE RADICAL BIO MED, V1, P331, DOI 10.1016/0748-5514(85)90140-0

[22] RECOGNITION OF OXIDIZED ABASIC SITES BY REPAIR ENDONUCLEASES [J].