EFFECT OF APHIDICOLIN ON DNA-SYNTHESIS IN ISOLATED HELA-CELL NUCLEI

被引：76

作者：

WIST, E ^{[1
]}

PRYDZ, H ^{[1
]}

机构：

[1] UNIV OSLO,FAC DENT,DEPT MICROBIOL,OSLO,NORWAY

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 04期

关键词：

D O I：

10.1093/nar/6.4.1583

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of the inhibitor aphidicolin on DNA synthesis in isolated nuclei from HeLa cells and on the activities of partially purified DNA polymerases has been tested. Aphidicolin inhibited DNA synthesis and DNA polymerase a very efficiently whereas DNA polymerases β and γ were insensitive to the drug. The results indicate that DNA polymerase α is the polymerase active during elongation as well as in the gapfilling process of discontinuous DNA synthesis. © 1979 Information Retrieval Limited.

引用

收藏

页码：1583 / 1590

页数：8

相关论文

共 17 条

[1] X-RAY CRYSTALLOGRAPHIC DETERMINATION OF STRUCTURE OF ANTIBIOTIC APHIDICOLIN - TETRACYCLIC DITERPENOID CONTAINING A NEW RING-SYSTEM [J].

BRUNDRET, KM ;

HESP, B ;

DALZIEL, W .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1972, (18) :1027-&

[2] ANTIVIRAL EFFECTS OF APHIDICOLIN, A NEW ANTIBIOTIC PRODUCED BY CEPHALOSPORIUM-APHIDICOLA [J].

BUCKNALL, RA ;

MOORES, H ;

SIMMS, R ;

HESP, B .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1973, 4 (03) :294-298

[3] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].

BURTON, K .

BIOCHEMICAL JOURNAL, 1956, 62 (02) :315-323

[4]

BUTT RT, 1978, BIOCH J, V173, P309

[5] ROLE OF DEOXYRIBONUCLEIC-ACID POLYMERASE BETA IN NUCLEAR DEOXYRIBONUCLEIC-ACID SYNTHESIS [J].

BUTT, TR ;

WOOD, WM ;

ADAMS, RLP .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1976, 4 (04) :807-810

[6]

EDENBERG HJ, 1978, J BIOL CHEM, V253, P3273

[7] APHIDICOLIN PREVENTS MITOTIC CELL-DIVISION BY INTERFERING WITH ACTIVITY OF DNA POLYMERASE-ALPHA [J].

IKEGAMI, S ;

TAGUCHI, T ;

OHASHI, M .

NATURE, 1978, 275 (5679) :458-460

[8] OKAZAKI PIECES GROW OPPOSITE TO REPLICATION FORK DIRECTION DURING SIMIAN-VIRUS 40-DNA REPLICATION [J].

KAUFMANN, G ;

BARSHAVIT, R ;

DEPAMPHILIS, ML .

NUCLEIC ACIDS RESEARCH, 1978, 5 (07) :2535-2545

[9] DNA-REPLICATION INTERMEDIATES IN WHOLE HELA-CELLS AND ISOLATED-NUCLEI [J].

KROKAN, H ;

WIST, E ;

PRYDZ, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 475 (04) :553-561

[10] DNA-SYNTHESIS IN ISOLATED HELA-CELL NUCLEI - EVIDENCE FOR INVITRO INITIATION OF SYNTHESIS OF SMALL PIECES OF DNA AND THEIR SUBSEQUENT LIGATION [J].

KROKAN, H ;

COOKE, L ;

PRYDZ, H .

BIOCHEMISTRY, 1975, 14 (19) :4233-4237