EFFECT OF APHIDICOLIN ON DNA-SYNTHESIS IN ISOLATED HELA-CELL NUCLEI

被引：76

作者：

WIST, E ^{[1
]}

PRYDZ, H ^{[1
]}

机构：

[1] UNIV OSLO,FAC DENT,DEPT MICROBIOL,OSLO,NORWAY

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 04期

关键词：

D O I：

10.1093/nar/6.4.1583

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of the inhibitor aphidicolin on DNA synthesis in isolated nuclei from HeLa cells and on the activities of partially purified DNA polymerases has been tested. Aphidicolin inhibited DNA synthesis and DNA polymerase a very efficiently whereas DNA polymerases β and γ were insensitive to the drug. The results indicate that DNA polymerase α is the polymerase active during elongation as well as in the gapfilling process of discontinuous DNA synthesis. © 1979 Information Retrieval Limited.

引用

页码：1583 / 1590

页数：8

共 17 条

[11] DNA-SYNTHESIS IN ISOLATED HELA-CELL NUCLEI - OPTIMALIZATION OF SYSTEM AND CHARACTERIZATION OF PRODUCT [J].