IDENTIFICATION OF THE PEROXIDATION PRODUCT HYDROXYSTEARIC ACID IN LEWIS LUNG-CARCINOMA CELLS

被引：14

作者：

CAVALLI, G ^{[1
]}

CASALI, E ^{[1
]}

SPISNI, A ^{[1
]}

MASOTTI, L ^{[1
]}

机构：

[1] DEPT BIOCHEM, I-40127 BOLOGNA, ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91029-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Whole cell lipids were extracted from the Lewis lung carcinoma in vitro line C108. The fatty acids were derivatized to methylesters in order to identify endogenous oxidized derivatives by gasmass spectroscopy. The presence of 9-hydroxystearic acid and 10-hydroxystearic acid was thus evidenced for the first time in cultured mammalian cells. Moreover a linear correlation was found between the concentration of these products expressed as percentage of total fatty acid methylesters and the cell density in tissue culture flasks. This finding suggests an involvement of hydroxystearic acid in cellular functions related to the cell density in monolayer cultures. © 1991.

引用

页码：1260 / 1265

页数：6

共 19 条

[1] STIMULATION OF DNA-SYNTHESIS AND MITOSIS OF HEPATOCYTES IN PRIMARY CULTURES OF NEONATAL RAT-LIVER BY ARACHIDONIC-ACID AND PROSTAGLANDINS [J].