DROSOPHILA POLYCOMB-GROUP REGULATED CHROMATIN INHIBITS THE ACCESSIBILITY OF A TRANSACTIVATOR TO ITS TARGET DNA

被引:173
作者
ZINK, D [1 ]
PARO, R [1 ]
机构
[1] UNIV HEIDELBERG,CTR MOLEC BIOL,ZMBH,D-69120 HEIDELBERG,GERMANY
关键词
BITHORAX COMPLEX; DROSOPHILA MELANOGASTER; GAL4; TRANSACTIVATOR; POLYCOMB; SILENCING;
D O I
10.1002/j.1460-2075.1995.tb00253.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The genes of the Polycomb-group (Pc-G) are responsible for maintaining the inactive expression state of homeotic genes, They act through specific cis-regulatory DNA elements termed PREs (Pc-G Response Elements). Multimeric complexes containing the Pc-G proteins are thought to induce heterochromatin-like structures, which stably and heritably inactivate transcription, We have tested the functional role of the FAB fragment, a PRE of the bithorax complex, We find that this element behaves as an orientation dependent silencer, capable of inducing mosaic gene expression on neighboring genes, Transgenic fly lines were constructed containing a PRE adjacent to a reporter gene inducible by the yeast GAL4 cans-activator. The competition between the activator and Pc-G-containing chromatin was visualized on polytene chromosomes using immunocytochemistry, The Pc-G protein Polycomb and GAL4 have mutually exclusive binding patterns, supporting the notion that Pc-G-induced chromatin structures can prevent activators from binding to their target sequences. However, this antagonistic function can be overcome by high doses of GAL4, even in the absence of DNA replication.
引用
收藏
页码:5660 / 5671
页数:12
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