AN ICE-LIKE PROTEASE IS A COMMON MEDIATOR OF APOPTOSIS INDUCED BY DIVERSE STIMULI IN HUMAN MONOCYTIC THP.1 CELLS

被引：146

作者：

ZHU, HJ ^{[1
]}

FEARNHEAD, HO ^{[1
]}

COHEN, GM ^{[1
]}

机构：

[1] UNIV LEICESTER, MRC, TOXICOL UNIT, LEICESTER LE1 9HN, LEICS, ENGLAND

来源：

FEBS LETTERS | 1995年 / 374卷 / 02期

关键词：

APOPTOSIS; ICE-LIKE PROTEASES; THP.1; CELL; Z-VAL-ALA-ASP FLUOROMETHYLKETONE;

D O I：

10.1016/0014-5793(95)01116-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apoptosis was induced in THP.1 cells, a human monocytic tumour cell line, by diverse stimuli including cyclo-heximide, thapsigargin, etoposide and staurosporine. Induction of apoptosis by all these stimuli, except etoposide, was enhanced in the presence of the trypsin-like protease inhibitor, N alpha-tosyl-L-lysinyl chloromethyl ketone (TLCK), Induction of apoptosis, assessed by morphological, flow cytometric and biochemical criteria, including proteolysis of poly(ADP-ribose) polymerase and cleavage of DNA to large kilobasepair fragments, was completely abrogated when cells were pretreated with an ICE-like protease inhibitor, Z-Val-Ala-Asp . fluoromethylketone. This suggested that an ICE homologue was a common mediator of apoptosis in THP.1 cells.

引用

页码：303 / 308

页数：6

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