QUANTITATIVE-ANALYSIS OF GROWTH AND REGENERATION FROM TISSUE-CULTURES OF HORDEUM-VULGARE, H JUBATUM AND THEIR INTERSPECIFIC HYBRID

被引：35

作者：

ORTON, TJ ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,PROGRAM GENET,E LANSING,MI 48824

来源：

ENVIRONMENTAL AND EXPERIMENTAL BOTANY | 1979年 / 19卷 / 04期

关键词：

D O I：

10.1016/0098-8472(79)90036-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A quantitative analysis of growth and regeneration was undertaken to identify significant media effects in tissue cultures of Hordeum vulgare (HV), H. jubatum (HJ) and their interspecific hybrid (HV×HJ). Immature ovaries generated the most vigorous and easily regenerated callus of several explanted tissues. After callus formation, cultures adapted one of five distinct types, characterized by differences in tissue consistency, pigmentation, growth rate and regeneration. Different growth rates were observed among subcalli of the same callus type. B5 medium supplemented with sucrose (1%), glucose (1%), kinetin (0.3 mg/l) and gibberellic acid (1 mg/l) maximized the regeneration of shoots and roots from certain callus cultures. Exogenously supplied indole-3-acetic acid (IAA)-amino acid conjugates promoted callus growth and inhibited regeneration more effectively than equivalent concentrations of free IAA, HV, HJ and HV×HJ exhibited similar patterns of media-induced callus growth and regeneration. © 1979.

引用

页码：319 / 335

页数：17

共 37 条

[31] DECLINE OF EMBRYOGENIC POTENTIAL AS CALLUS AND SUSPENSION CULTURES OF CARROT (DAUCUS-CAROTA L) ARE SERIALLY SUBCULTURED
SMITH, SM
STREET, HE
[J]. ANNALS OF BOTANY, 1974, 38 (155) : 223 - +
[32] STEIDL RP, 1976, THESIS MICHIGAN STAT
[33] Sunderland N, 1977, PLANT TISSUE CELL CU, P177
[34] MORPHOGENETIC POTENTIAL OF CULTURED LEAF SECTIONS OF CULTIVATED AND WILD-SPECIES OF TOMATO
TAL, M
DEHAN, K
HEIKIN, H
[J]. ANNALS OF BOTANY, 1977, 41 (175) : 937 - 941
[35] MORPHOGENESIS IN RELATION TO CHROMOSOMAL CONSTITUTION IN LONG-TERM PLANT TISSUE CULTURES
TORREY, JG
[J]. PHYSIOLOGIA PLANTARUM, 1967, 20 (02) : 265 - +
[36] VASIL IK, 1972, IN VITRO CELL DEV B, V8, P117
[37] Yamada Y., 1977, Plant cell, tissue and organ culture. Chapter I. Regeneration of plants, vegetative propagation and cloning., P144

← 1 2 3 4 →