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KUNITZ-TYPE TRYPSIN-INHIBITOR PREVENTS LPS-INDUCED INCREASE OF CYTOSOLIC-FREE CA2+ IN HUMAN NEUTROPHILS AND HUVEC CELLS

被引：30
作者：
KANAYAMA, N
HALIM, A
MAEHARA, K
KAJIWARA, Y
FUJIE, M
TERAO, T
机构：
[1] Department Obstetrics and Gynecology, Hamamatsu University School Medicine, Hamamatsu 431-31
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 207卷 / 01期
关键词：
D O I：
10.1006/bbrc.1995.1191
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The protease inhibitor part of inter-alpha trypsin inhibitor is identical to urinary trypsin inhibitor (UTI). Preincubation of neutrophils and HUVEC cells with UTI inhibited increase of cytosolic free Ca2+ induced by LPS. Increase of cytosolic free Ca2+ induced by LPS in the presence of EGTA was also inhibited by UTI. In contrast, UTI did not inhibit increase of cytosolic free Ca2+ in cells stimulated by Ca2+ ionophore with or without EGTA. The effects of nine synthetic peptides of UTI on the concentration of cytosolic free Ca2+ in the neutrophils induced by LPS were examined. Preincubation with a peptide of UTI domain 2, NLPIVRGPCRAFIQL (83-97), was completely inhibited by the increase of cytosolic free Ca2+ in neutrophils. This region is identical to the trypsin inhibitor site of UTI. We propose that a function of UTI other than as a protease inhibitor is in regulation of intracellular Ca2+ and that this is due to its trypsin inhibitor region. (C) 1995 Academic Press, Inc.
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页码：324 / 330
页数：7
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