DIRECT DEMONSTRATION OF GUANINE-NUCLEOTIDE SENSITIVE RECEPTORS FOR VASOACTIVE-INTESTINAL-PEPTIDE IN THE ANTERIOR LOBE OF THE RAT PITUITARY-GLAND

被引：5

作者：

AGUI, T ^{[1
]}

MATSUMOTO, K ^{[1
]}

机构：

[1] NIAID,IMMUNOL LAB,BETHESDA,MD 20892

来源：

PEPTIDES | 1990年 / 11卷 / 03期

关键词：

Guanine nucleotide; Pituitary gland; Prolactin; VIP receptors;

D O I：

10.1016/0196-9781(90)90065-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The vasoactive intestinal peptide (VIP) receptors were identified on the membranes from the rat anterior pituitary gland with [125I]VIP. The dissociation constant (Kd) and the maximal binding capacity (Bmax) values were estimated from the competitive inhibition data. The Kd and Bmax values were 1.05±0.75 nM and 103±11 fmol/mg protein, respectively. The order of molar potency of related peptides to inhibit [125I]VIP binding was VIP > peptide histidine isoleucine (PHI) > secretin > glucagon. Glucagon was not effective to inhibit the binding. [125I]VIP binding was effectively inhibited by the addition of guanine nucleotides. The order of molar potency to inhibit the binding was Gpp(NH)p > GTP > GDP > GMP > ATP. These results directly suggest the coupling of VIP receptors with guanine nucleotide binding proteins in the anterior pituitary gland. © 1990.

引用

页码：609 / 611

页数：3

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