COLUMN LIQUID-CHROMATOGRAPHY IN COMBINATION WITH IMMOBILIZED ENZYMES AND ELECTROCHEMICAL DETECTION AND ITS APPLICATIONS IN SOME INDUSTRIAL-PROCESSES

The first part of the paper provides a review of the pre- and postcolumn derivatization systems used in Column Liquid Chromatography (CLC) in combination with immobilized enzyme reactors (IMERs) and electrochemical detection (EC). In the second part, an outline of important factors to consider in the optimization of CLC-IMER-EC systems are presented. Three industrial applications are described utilizing enzyme-based detection or enzymes for sample handling purposes. In two of the cases, CLC-IMER were used in combination with amperometric detection using chemically modified electrodes (CME). These applications were performed in our laboratory, and the high selectivity and sensitivity of these systems as well as the problems encountered in these complex samples will be discussed.