CATALYSIS OF ENZYME AGGREGATES IN ORGANIC-SOLVENTS - AN ATTEMPT AT EVALUATION OF INTRINSIC ACTIVITY OF PROTEASES IN ETHANOL

被引：15

作者：

YAMAMOTO, Y ^{[1
]}

KISE, H ^{[1
]}

机构：

[1] UNIV TSUKUBA,INST MAT SCI,TSUKUBA,IBARAKI 305,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 06期

关键词：

D O I：

10.1007/BF00138557

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Catalytic activities of alpha-chymotrypsin and subtilisin Carlsberg for transesterification of N-acetyl-L-tyrosine methyl ester in ethanol markedly increased by decreasing the amount of the enzymes in the reaction mixtures. The results were kinetically attributed to changes in K(M) and k(cat) due to formation of smaller enzyme aggregates.

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页码：647 / 652

页数：6

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