DETECTION OF ESTROGEN-LIKE AND DIOXIN-LIKE ACTIVITY IN PULP AND PAPER-MILL BLACK LIQUOR AND EFFLUENT USING IN-VITRO RECOMBINANT RECEPTOR REPORTER GENE ASSAYS

被引：93

作者：

ZACHAREWSKI, TR ^{[1
]}

BERHANE, K ^{[1
]}

GILLESBY, BE ^{[1
]}

BURNISON, BK ^{[1
]}

机构：

[1] NATL WATER RES INST BRANCH,AQUAT ECOSYST CONSERVAT BRANCH,BURLINGTON,ON L7R 4A6,CANADA

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 1995年 / 29卷 / 08期

关键词：

D O I：

10.1021/es00008a037

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In vitro recombinant receptor/reporter gene assays were used to examine pulp and paper mill black liquor and effluent for estrogenic, dioxin-like, and antiestrogenic activities. Using MCF-7 cells transiently transfected with a Gal4-estrogen receptor chimeric construct (Gal4-HEGO) and a Gal4-regulated luciferase reporter gene (17m5-G-Luc), it was estimated that black liquor contains 4 +/- 2 ppb ''estrogen equivalents'', while negligible estrogenic activity was observed in a methanol-extracted pulp and paper mill effluent fraction (MF). A dioxin response element (DRE)-regulated luciferase reporter gene (pGudLuc1.1) transiently transfected into Hepa1c1c7 wild-type cells exhibited a dose-dependent increase in luciferase activity following treatment with 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD), black liquor, and MF. Based on the dose-response curves, black liquor and MF contain 10 +/- 4 ppb and 20 +/- 6 ppt ''TCDD equivalents'', respectively. Moreover, MF exhibited significant AhR-mediated antiestrogenic activity. These results demonstrate the utility of these bioassays and suggest that the effects observed in fish exposed to pulp and paper mill effluent may be due to unidentified ER and AhR ligands not detected by conventional chemical analysis due to the lack of appropriate standards.

引用

页码：2140 / 2146

页数：7

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