MEKC ANALYSIS OF FITC AND DTAF AMINO-ACID DERIVATIVES WITH LIF DETECTION

被引：30

作者：

LALLJIE, SPD ^{[1
]}

SANDRA, P ^{[1
]}

机构：

[1] STATE UNIV GHENT,DEPT ORGAN CHEM,B-9000 GHENT,BELGIUM

来源：

CHROMATOGRAPHIA | 1995年 / 40卷 / 9-10期

关键词：

MICELLAR ELECTROKINETIC CHROMATOGRAPHY; RESOLUTION OPTIMIZATION; MIXED MICELLES; ORGANIC MODIFIERS;

D O I：

10.1007/BF02290261

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of micellar electrokinetic chromatography to the analysis of complex mixtures of FITC and DTAF amino acid derivatives was studied. Resolution optimization was attempted for a mixture of 21 amino acids. In the case of FITC, by increasing the analysis temperature to 35 degrees C, a total of 17 FITC amino acids could be separated. On the other hand, by using a combination of mixed micelles (SDS/Brij-35) and the addition of organic modifiers, a total of 19 DTAF derivatized amino acids could be separated.

引用

页码：513 / 518

页数：6

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