MEKC ANALYSIS OF FITC AND DTAF AMINO-ACID DERIVATIVES WITH LIF DETECTION

被引：30

作者：

LALLJIE, SPD ^{[1
]}

SANDRA, P ^{[1
]}

机构：

[1] STATE UNIV GHENT,DEPT ORGAN CHEM,B-9000 GHENT,BELGIUM

来源：

CHROMATOGRAPHIA | 1995年 / 40卷 / 9-10期

关键词：

MICELLAR ELECTROKINETIC CHROMATOGRAPHY; RESOLUTION OPTIMIZATION; MIXED MICELLES; ORGANIC MODIFIERS;

D O I：

10.1007/BF02290261

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of micellar electrokinetic chromatography to the analysis of complex mixtures of FITC and DTAF amino acid derivatives was studied. Resolution optimization was attempted for a mixture of 21 amino acids. In the case of FITC, by increasing the analysis temperature to 35 degrees C, a total of 17 FITC amino acids could be separated. On the other hand, by using a combination of mixed micelles (SDS/Brij-35) and the addition of organic modifiers, a total of 19 DTAF derivatized amino acids could be separated.

引用

页码：513 / 518

页数：6

共 32 条

[21] STRATEGIES FOR THE MONITORING OF DRUGS IN BODY-FLUIDS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY [J].

THORMANN, W ;

LIENHARD, S ;

WERNLY, P .

JOURNAL OF CHROMATOGRAPHY, 1993, 636 (01) :137-148

[22] SEPARATION OF NAPHTHALENE-2,3-DICARBOXALDEHYDE-LABELED AMINO-ACIDS BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION [J].

UEDA, T ;

MITCHELL, R ;

KITAMURA, F ;

METCALF, T ;

KUWANA, T ;

NAKAMOTO, A .

JOURNAL OF CHROMATOGRAPHY, 1992, 593 (1-2) :265-274

[23]

VANDEGOOR TAA, 1992, THESIS EINDHOVEN U T

[24]

VINDEVOGEL J, 1992, INTRO MICELLAR ELECT

[25] CAPILLARY ZONE ELECTROPHORESIS SEPARATION AND LASER-BASED DETECTION OF BOTH FLUORESCEIN THIOHYDANTOIN AND DIMETHYLAMINOAZOBENZENE THIOHYDANTOIN DERIVATIVES OF AMINO-ACIDS [J].

WALDRON, KC ;

WU, SL ;

EARLE, CW ;

HARKE, HR ;

DOVICHI, NJ .

ELECTROPHORESIS, 1990, 11 (09) :777-780

[26]

WALLINGFORD RA, 1989, J MICROCOLUMN SEP, V1, P23

[27]

WANDERS BJ, 1993, THESIS EINDHOVEN U T

[28] THE SEPARATION OF HERBICIDES BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY [J].

WU, Q ;

CLAESSENS, HA ;

CRAMERS, CA .

CHROMATOGRAPHIA, 1992, 34 (1-2) :25-30

[29] CAPILLARY ZONE ELECTROPHORESIS SEPARATION AND LASER-INDUCED FLUORESCENCE DETECTION OF ZEPTOMOLE QUANTITIES OF FLUORESCEIN THIOHYDANTOIN DERIVATIVES OF AMINO-ACIDS [J].

WU, SO ;

DOVICHI, NJ .

TALANTA, 1992, 39 (02) :173-178

[30] RESOLUTION OPTIMIZATION IN MICELLAR ELECTROKINETIC CHROMATOGRAPHY - USE OF OVERLAPPING RESOLUTION MAPPING SCHEME IN THE ANALYSIS OF DINITROPHENYL DERIVATIZED AMINO-ACIDS [J].

YIK, YF ;

LI, SFY .

CHROMATOGRAPHIA, 1993, 35 (9-12) :560-566

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