STRAIN IMPROVEMENT OF PHAFFIA-RHODOZYMA BY PROTOPLAST FUSION

被引：30

作者：

CHUN, SB ^{[1
]}

CHIN, JE ^{[1
]}

BAI, S ^{[1
]}

AN, GH ^{[1
]}

机构：

[1] HAI TAI CONFECT CO,HAI TAI FOOD RES INST,SEOUL,SOUTH KOREA

来源：

FEMS MICROBIOLOGY LETTERS | 1992年 / 93卷 / 03期

关键词：

PROTOPLAST FUSION; PHAFFIA-RHODOZYMA; STRAIN IMPROVEMENT; CAROTENOID; ASTAXANTHIN;

D O I：

10.1016/0378-1097(92)90466-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The application of Phaffia rhodozyma as an astaxanthin source in the aquaculture industry is limited because of the low carotenoid content of natural isolates. We constructed carotenoid hyperproducing hybrids of P. rhodozyma by protoplast fusion from parental strains that produced approx. 1600-mu-g carotenoid/g yeast and were auxotrophic for tryptophan, leucine, methionine or arginine. The hybrids were stable and consistently produced > 2000-mu-g carotenoid/g yeast. Karyogamy was confirmed by the isolation of recombinants after mitotic segregation of parental auxotrophic genetic markers, the increased content of DNA/cell and the presence of a single nucleus per cell. The results of this study indicated that protoplast fusion is promising for strain improvement in P. rhodozyma.

引用

页码：221 / 226

页数：6

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