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PURIFICATION AND CHARACTERIZATION OF BOVINE BRAIN TYPE-I PHOSPHATIDYLINOSITOL KINASE

被引：119
作者：
MORGAN, SJ
SMITH, AD
PARKER, PJ
机构：
[1] LUDWIG INST CANC RES,91 RIDING HOUSE ST,LONDON W1P 8BT,ENGLAND
[2] UNIV COLL & MIDDLESEX SCH MED,DEPT CHEM PATHOL,LONDON,ENGLAND
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 191卷 / 03期
关键词：
D O I：
10.1111/j.1432-1033.1990.tb19185.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Investigation into the phosphatidylinositol kinase activities in bovine brain has revealed the presence of a type I PtdIns kinase activity. This classification is based upon potent inhibition by neutral detergent and the production of a phosphatidylinositol phosphate that can be distinguished from phosphatidyl‐inositol 4‐phosphate [PtdIns(4)P] by thin‐layer chromatography. The enzyme has been substantially purified and the activity is associated with an 85‐kDa polypeptide on SDS/polyacrylamide gel electrophoresis. Analysis of the product confirms the identification of the enzyme as a type I PtdIns kinase. The purified kinase has been characterized with respect to substrate dependence (Mg2+, ATP, PtdIns), substrate presentation (pure lipid versus mixed micelle) and specificity [PtdIns versus PtdIns(4)P and phosphatidylinositol 4,5‐bisphosphate]. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：761 / 767
页数：7
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