PURIFICATION AND CHARACTERIZATION OF BOVINE BRAIN TYPE-I PHOSPHATIDYLINOSITOL KINASE

被引：119

作者：

MORGAN, SJ

SMITH, AD

PARKER, PJ

机构：

[1] LUDWIG INST CANC RES,91 RIDING HOUSE ST,LONDON W1P 8BT,ENGLAND

[2] UNIV COLL & MIDDLESEX SCH MED,DEPT CHEM PATHOL,LONDON,ENGLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 191卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb19185.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Investigation into the phosphatidylinositol kinase activities in bovine brain has revealed the presence of a type I PtdIns kinase activity. This classification is based upon potent inhibition by neutral detergent and the production of a phosphatidylinositol phosphate that can be distinguished from phosphatidyl‐inositol 4‐phosphate [PtdIns(4)P] by thin‐layer chromatography. The enzyme has been substantially purified and the activity is associated with an 85‐kDa polypeptide on SDS/polyacrylamide gel electrophoresis. Analysis of the product confirms the identification of the enzyme as a type I PtdIns kinase. The purified kinase has been characterized with respect to substrate dependence (Mg2+, ATP, PtdIns), substrate presentation (pure lipid versus mixed micelle) and specificity [PtdIns versus PtdIns(4)P and phosphatidylinositol 4,5‐bisphosphate]. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：761 / 767

页数：7

共 31 条

[21] PURIFICATION OF PHOSPHATIDYLINOSITOL KINASE FROM BOVINE BRAIN MYELIN
SALTIEL, AR
FOX, JA
SHERLINE, P
SAHYOUN, N
CUATRECASAS, P
[J]. BIOCHEMICAL JOURNAL, 1987, 241 (03) : 759 - 763
[22] METABOLIC AND STRUCTURAL EVIDENCE FOR THE EXISTENCE OF A 3RD-SPECIES OF POLYPHOSPHOINOSITIDE IN CELLS - D-PHOSPHATIDYL-MYO-INOSITOL 3-PHOSPHATE
STEPHENS, L
HAWKINS, PT
DOWNES, CP
[J]. BIOCHEMICAL JOURNAL, 1989, 259 (01) : 267 - 276
[23] RELEASE OF CA-2+ FROM A NONMITOCHONDRIAL INTRACELLULAR STORE IN PANCREATIC ACINAR-CELLS BY INOSITOL-1,4,5-TRISPHOSPHATE
STREB, H
IRVINE, RF
BERRIDGE, MJ
SCHULZ, I
[J]. NATURE, 1983, 306 (5938) : 67 - 69
[24] EVIDENCE THAT THE ROUS-SARCOMA VIRUS TRANSFORMING GENE-PRODUCT PHOSPHORYLATES PHOSPHATIDYLINOSITOL AND DIACYLGLYCEROL
SUGIMOTO, Y
WHITMAN, M
CANTLEY, LC
ERIKSON, RL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07): : 2117 - 2121
[25] UNSATURATED DIACYLGLYCEROL AS A POSSIBLE MESSENGER FOR THE ACTIVATION OF CALCIUM-ACTIVATED, PHOSPHOLIPID DEPENDENT PROTEIN-KINASE SYSTEM
TAKAI, Y
KISHIMOTO, A
KIKKAWA, U
MORI, T
NISHIZUKA, Y
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 91 (04) : 1218 - 1224
[26] AN INOSITOL TETRAKISPHOSPHATE-CONTAINING PHOSPHOLIPID IN ACTIVATED NEUTROPHILS
TRAYNORKAPLAN, AE
HARRIS, AL
THOMPSON, BL
TAYLOR, P
SKLAR, LA
[J]. NATURE, 1988, 334 (6180) : 353 - 356
[27] THE COLONY STIMULATING FACTOR-I RECEPTOR ASSOCIATES WITH AND ACTIVATES PHOSPHATIDYLINOSITOL-3 KINASE
VARTICOVSKI, L
DRUKER, B
MORRISON, D
CANTLEY, L
ROBERTS, T
[J]. NATURE, 1989, 342 (6250) : 699 - 702
[28] PURIFICATION AND CHARACTERIZATION OF A PHOSPHATIDYLINOSITOL KINASE FROM A431 CELLS
WALKER, DH
DOUGHERTY, N
PIKE, LJ
[J]. BIOCHEMISTRY, 1988, 27 (17) : 6504 - 6511
[29] EVIDENCE FOR 2 DISTINCT PHOSPHATIDYLINOSITOL KINASES IN FIBROBLASTS - IMPLICATIONS FOR CELLULAR-REGULATION
WHITMAN, M
KAPLAN, D
ROBERTS, T
CANTLEY, L
[J]. BIOCHEMICAL JOURNAL, 1987, 247 (01) : 165 - 174
[30] TYPE-I PHOSPHATIDYLINOSITOL KINASE MAKES A NOVEL INOSITOL PHOSPHOLIPID, PHOSPHATIDYLINOSITOL-3-PHOSPHATE
WHITMAN, M
DOWNES, CP
KEELER, M
KELLER, T
CANTLEY, L
[J]. NATURE, 1988, 332 (6165) : 644 - 646

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