AN INHIBITOR OF INOSITOL-PHOSPHOLIPID-SPECIFIC PHOSPHOLIPASE-C

被引：18

作者：

OGAWARA, H

HIGASHI, K

MANITA, S

HIDAKA, M

KATO, H

TAKENAWA, T

机构：

[1] KUMAMOTO UNIV,SCH MED,DEPT INTERNAL MED 2,KUMAMOTO 860,JAPAN

[2] TOKYO METROPOLITAN GERIATR HOSP & INST GERONTOL,DEPT BIOSIGNAL RES,ITABASHI KU,TOKYO 173,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1175卷 / 03期

关键词：

POLYPHOSPHOINOSITIDE; PHOSPHOLIPASE-C; INHIBITOR; ACTINOMADURA;

D O I：

10.1016/0167-4889(93)90219-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Q12713 substance, a cyclic peptide from Actinomadura spp., strongly inhibited the enzyme activity of phosphatidylinositol-4,5-bisphosphate-specific phospholipase C (PIP2-PLC) but scarcely inhibited other phospholipases. Kinetic analysis demonstrated that the inhibition of PIP2-PLC activity was competitive with respect to phosphatidylinositol 4,5-bisphosphate. Calcium and magnesium ions had no significant effect on the inhibitory activity. On the contrary, potassium or rubidium ion was essential for the inhibitory activity. Furthermore, NaF and AlCl3-stimulated increase of phosphoinositides was decreased by Q12713 in the cultured 3T3 cells.

引用

页码：289 / 292

页数：4

共 16 条

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