PHOSPHATIDYLINOSITOL 3-KINASE - STRUCTURE AND EXPRESSION OF THE 110KD CATALYTIC SUBUNIT

被引：639

作者：

HILES, ID

OTSU, M

VOLINIA, S

FRY, MJ

GOUT, I

DHAND, R

PANAYOTOU, G

RUIZLARREA, F

THOMPSON, A

TOTTY, NF

HSUAN, JJ

COURTNEIDGE, SA

PARKER, PJ

WATERFIELD, MD

机构：

[1] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOLEC BIOL,LONDON WC1E 6BT,ENGLAND

[2] EUROPEAN MOLEC BIOL LAB,DIFFERENTIAT PROGRAMME,W-6900 HEIDELBERG,GERMANY

[3] IMPERIAL CANC RES FUND,LONDON WC2A 3PX,ENGLAND

来源：

CELL | 1992年 / 70卷 / 03期

关键词：

D O I：

10.1016/0092-8674(92)90166-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Purified bovine brain phosphatidylinositol 3-kinase (Pl3-kinase) is composed of 85 kd and 110 kd subunits. The 85 kd subunit (p85-alpha) lacks Pl3-kinase activity and acts as an adaptor, coupling the 110 kd subunit (p110) to activated protein tyrosine kinases. Here the characterization of the p110 subunit is presented. cDNA cloning reveals p110 to be a 1068 aa protein related to Vps34p, a S. cerevisiae protein involved in the sorting of proteins to the vacuole. p110 expressed in insect cells possesses Pl3-kinase activity and associates with p85-alpha into an active p85-alpha-p110 complex that binds the activated colony-stimulating factor 1 receptor. p110 expressed in COS-1 cells is catalytically active only when complexed with p85-alpha.

引用

收藏

页码：419 / 429

页数：11

相关论文

共 63 条

[21] CHARACTERIZATION OF VPS34, A GENE REQUIRED FOR VACUOLAR PROTEIN SORTING AND VACUOLE SEGREGATION IN SACCHAROMYCES-CEREVISIAE [J].

HERMAN, PK ;

EMR, SD .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (12) :6742-6754

[22] INTERACTION OF PHOSPHATIDYLINOSITOL 3-KINASE-ASSOCIATED P85 WITH EPIDERMAL GROWTH-FACTOR AND PLATELET-DERIVED GROWTH-FACTOR RECEPTORS [J].

HU, P ;

MARGOLIS, B ;

SKOLNIK, EY ;

LAMMERS, R ;

ULLRICH, A ;

SCHLESSINGER, J .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (03) :981-990

[23] PHOSPHATIDYLINOSITOL METABOLISM AND POLYOMA-MEDIATED TRANSFORMATION [J].

KAPLAN, DR ;

WHITMAN, M ;

SCHAFFHAUSEN, B ;

RAPTIS, L ;

GARCEA, RL ;

PALLAS, D ;

ROBERTS, TM ;

CANTLEY, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) :3624-3628

[24] COMMON ELEMENTS IN GROWTH-FACTOR STIMULATION AND ONCOGENIC TRANSFORMATION - 85-KD PHOSPHOPROTEIN AND PHOSPHATIDYLINOSITOL KINASE-ACTIVITY [J].

KAPLAN, DR ;

WHITMAN, M ;

SCHAFFHAUSEN, B ;

PALLAS, DC ;

WHITE, M ;

CANTLEY, L ;

ROBERTS, TM .

CELL, 1987, 50 (07) :1021-1029

[25] THE PHOSPHORYLATION STATE OF EUKARYOTIC INITIATION FACTOR-II ALTERS TRANSLATIONAL EFFICIENCY OF SPECIFIC MESSENGER-RNAS [J].

KAUFMAN, RJ ;

DAVIES, MV ;

PATHAK, VK ;

HERSHEY, JWB .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (03) :946-958

[26] PHOSPHORYLATION OF THE PDGF RECEPTOR BETA-SUBUNIT CREATES A TIGHT-BINDING SITE FOR PHOSPHATIDYLINOSITOL-3 KINASE [J].

KAZLAUSKAS, A ;

COOPER, JA .

EMBO JOURNAL, 1990, 9 (10) :3279-3286

[27] AUTOPHOSPHORYLATION OF THE PDGF RECEPTOR IN THE KINASE INSERT REGION REGULATES INTERACTIONS WITH CELL-PROTEINS [J].

KAZLAUSKAS, A ;

COOPER, JA .

CELL, 1989, 58 (06) :1121-1133

[28] FUNCTIONS OF THE MAJOR TYROSINE PHOSPHORYLATION SITE OF THE PDGF RECEPTOR BETA-SUBUNIT [J].

KAZLAUSKAS, A ;

DURDEN, DL ;

COOPER, JA .

CELL REGULATION, 1991, 2 (06) :413-425

[29]

KELLY KL, 1992, J BIOL CHEM, V267, P3423

[30] CRYSTAL-STRUCTURE OF THE CATALYTIC SUBUNIT OF CYCLIC ADENOSINE-MONOPHOSPHATE DEPENDENT PROTEIN-KINASE [J].

KNIGHTON, DR ;

ZHENG, JH ;

TENEYCK, LF ;

ASHFORD, VA ;

XUONG, NH ;

TAYLOR, SS ;

SOWADSKI, JM .

SCIENCE, 1991, 253 (5018) :407-414

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