CARBOHYDRATE CONTENT OF ACID ALPHA-GLUCOSIDASE (GAMMA-AMYLASE) FROM HUMAN-LIVER

被引：13

作者：

BELENKY, DM ^{[1
]}

MIKHAJLOV, VI ^{[1
]}

ROSENFELD, EL ^{[1
]}

机构：

[1] ACAD MED SCI USSR, INST BIOL & MED CHEM, MOSCOW 119117, USSR

来源：

CLINICA CHIMICA ACTA | 1979年 / 93卷 / 03期

关键词：

D O I：

10.1016/0009-8981(79)90286-9

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The presence of carbohydrates in homogeneous preparations of human liver acid .alpha.-glucosidase (EC 3.2.1.3) was established and the carbohydrate content of the enzyme determined. The enzyme was purified with the specific purpose of removing all low MW carbohydrates. It was specifically adsorbed on concanavalin A-Sepharose, eluted with methyl-.alpha.-D-mannopyranoside and gave a positive reaction with the phenol-sulphuric acid reagent, showing that the enzyme studied is a glycoprotein. There were 8.3 glucosamine, 13.2 mannose and possibly 3-4 glucose residues per molecule of the enzyme with a MW of 98,000.

引用

页码：365 / 370

页数：6