METABOLISM OF (+)-ABSCISIC ACID TO DIHYDROPHASEIC-ACID-4'-BETA-D-GLUCOPYRANOSIDE BY SUNFLOWER EMBRYOS

被引：10

作者：

BARTHE, P ^{[1
]}

HOGGE, LR ^{[1
]}

ABRAMS, SR ^{[1
]}

LEPAGEDEGIVRY, MT ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,INST PLANT BIOTECHNOL,SASKATOON S7N 0W9,SASKATCHEWAN,CANADA

来源：

PHYTOCHEMISTRY | 1993年 / 34卷 / 03期

关键词：

HELIANTHUS-ANNUUS; COMPOSITAE; SUNFLOWER; (+) ABSCISIC ACID METABOLISM; DIHYDROPHASEIC ACID GLUCOSIDE; EMBRYO DORMANCY; CHIRAL HPLC;

D O I：

10.1016/0031-9422(93)85332-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(+)-[2-C-14]Abscisic acid [(+) ABA], prepared from (+/-)-[2-C-14] ABA by HPLC on a chiral column, at an initial concentration of 10 muM, was absorbed and metabolized very quickly by sunflower embryos. Oxidative products showed a small transient accumulation as their free forms after an incubation of 16 hr; at this time they accounted for about 16% of the total radioactivity absorbed. Whatever the incubation time, their esterified forms were detected only in small amounts. The major metabolite which accumulated with incubation time was acidic, of a highly polar nature and resistant to hydrolysis. It was isolated by reversed phase HPLC, HPTLC and TLC and characterized by mass spectrometry as dihydrophaseic acid glucopyranoside. Such a conjugate of an oxidative catabolite could not play the role of a potential source of ABA upon hydration. So our results do not support the hypothesis of an eventual involvement of ABA conjugates in the maintenance of embryo dormancy in sunflower.

引用

页码：645 / 648

页数：4

共 18 条

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