PROCESSING ENDOPEPTIDASE DEFICIENCY IN NEUROHYPOPHYSEAL SECRETORY GRANULES OF THE DIABETES-INSIPIDUS (BRATTLEBORO) RAT

被引:5
作者
CHAUVET, J [1 ]
ROUILLE, Y [1 ]
SPANG, A [1 ]
CARDINE, AM [1 ]
ACHER, R [1 ]
机构
[1] UNIV PARIS 06,BIOL CHEM LAB,96 BLVD RASPAIL,F-75005 PARIS,FRANCE
关键词
DIABETES-INSIPIDUS (BRATTLEBORO) RAT; DIBASIC PROCESSING ENDOPEPTIDASES; NEUROSECRETORY GRANULES; NEUROHYPOPHYSEAL HORMONES; PROVASOPRESSIN PROCESSING;
D O I
10.1007/BF01122032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The homozygote Brattleboro rat exhibits a hereditary diabetes insipidus due to a deficiency of vasopressin, the antidiuretic hormone. It has previously been shown that in this animal a single nucleotide deletion in the provasopressin gene leads to a mutant precursor with a C-terminal amino acid sequence different from that of the wild-type. However the N-terminal region including the hormone moiety, the processing signal as well as the first two-thirds of the neurophysin is entirely preserved and absence of maturation has to be explained by an additional cause. We show here that the neurohypophysis of the homozygote Brattleboro rat, in contrast to the adenohypophysis, displays a significant decrease in the Lys-Arg processing endopeptidase activity when compared to the heterozygote or the wild-type Wistar. It is suggested that hypothalamic vasopressinergic neurons of the homozygote Brattleboro rat display a deficiency in the processing enzyme in contrast to the oxytocinergic neurons in which processing of prooxytocin is normal.
引用
收藏
页码:445 / 451
页数:7
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