OPIOID-INDUCED INHIBITION OF VOLTAGE-GATED CALCIUM CHANNELS PARALLELS EXPRESSION OF OMEGA-CONOTOXIN-SENSITIVE CHANNEL SUBTYPE DURING DIFFERENTIATION OF NG108-15 CELLS

被引：25

作者：

JIN, WZ ^{[1
]}

LEE, NM ^{[1
]}

LOH, HH ^{[1
]}

THAYER, SA ^{[1
]}

机构：

[1] UNIV MINNESOTA,SCH MED,DEPT PHARMACOL,3-249 MILLARD HALL,435 DELAWARE ST,MINNEAPOLIS,MN 55455

来源：

BRAIN RESEARCH | 1993年 / 607卷 / 1-2期

关键词：

OPIOID; CA-2+ CHANNEL; OMEGA-CONOTOXIN; DADLE; DIFFERENTIATION; NG108-15;

D O I：

10.1016/0006-8993(93)91484-A

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Depolarization-induced increases in intracellular free calcium concentration ([Ca2+]i) were measured in single NG108-15 cells using indo-1 based microfluorimetry. In cells differentiated for 6 days in serum-free forskolin (5 muM) supplemented media, application of micromoiar concentrations Of [D-Ala2-D-Leu5]enkephalin (DADLE) inhibited Ca2+ influx mediated by voltage-gated Ca2+ channels. Inhibition of 50 mM K+-induced Ca2+ influx by DADLE was concentration-dependent over the range of 0.1 to 10 muM and blocked by 100 muM naloxone. Differentiation increased the amplitude of depolarization-induced [Ca2+]i transients from 78 +/- 21 nM in undifferentiated cells to 1,282 +/- 318 nM after 6 days. One muM nitrendipine inhibited Ca2+ influx by at least 65% at all stages of differentiation, while sensitivity to omega-conotoxin GVIa (omega-CgTx) did not appear until day 3. Omega-CgTx inhibited a dihydropyridine-sensitive Ca2+ channel. DADLE inhibition of Ca2+ channels did not appear until 3 days of differentiation. Thus, opioid inhibition of depolarization-induced Ca2+ influx paralleled the expression of omega-CgTx sensitive voltage-gated Ca2+ channels.

引用

页码：17 / 22

页数：6

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