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CHOLINE-SENSING ELECTRODE BASED ON POLYETHYLENE GLYCOL-MODIFIED ENZYME AND MEDIATOR

被引：19
作者：
YABUKI, S
MIZUTANI, F
KATSURA, T
机构：
[1] National Institute of Bioscience and Human-technology, Tsukuba, Ibaraki, 305
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 1994年 / 20卷 / 2-3期
关键词：
D O I：
10.1016/0925-4005(94)01186-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Polyethylene glycol-modified choline oxidase (PEG-ChOD) has been prepared in order to construct choline-sensing carbon-paste electrodes: the PEG-ChOD and mediator, 1,1'-dimethylferrocene, are incorporated into a carbon-paste matrix. The PEG-ChOD-based electrode exhibits higher enzyme activity than an electrode using native ChOD, since the PEG is effective in protecting ChOD from oil in the paste. The current response of the PEG-ChOD-based electrode is larger than that of the native ChOD-based one for choline concentrations higher than 5 mM. The PEG-ChOD-based electrode is more stable than the native ChOD-based one. Coating the surface of the PEG-ChOD-based electrode with a cation polymer is effective in enhancing the current response to choline: the current response of the PEG-ChOD-based electrode coated with a layer of AQ-29D (Kodak) is about four times larger than that of the AQ-29D-free electrode.
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页码：159 / 162
页数：4
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