POSITIONAL SPECIFICITY OF KETODIENES FROM LINOLEIC-ACID AEROBICALLY FORMED BY LIPOXYGENASE ISOZYMES FROM KIDNEY BEAN AND PEA

被引：34

作者：

SANZ, LC ^{[1
]}

PEREZ, AG ^{[1
]}

RIOS, JJ ^{[1
]}

OLIAS, JM ^{[1
]}

机构：

[1] CSIC,INST GRASA & DERIVADOS,UEI FISIOL & TECNOL POSTRECOLECC,AVE PADRE GARCIA TEJERO 4,E-41012 SEVILLE,SPAIN

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1993年 / 41卷 / 05期

关键词：

D O I：

10.1021/jf00029a002

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Two lipoxygenase isozymes (LOX-1 and LOX-2) from kidney bean and pea flours were purified by a combination of ammonium sulfate fractionation, gel filtration, and ion-exchange chromatography. Besides the production of hydroperoxides, LOX-1 catalyzed the aerobic formation of ketodienes. The positional and geometrical isomers were separated and quantified by HPLC. LOX-1 yielded approximately equal proportions of 9- and 13-hydroperoxides and the 9- and 13-ketodienes; LOX-2 formed mainly the 13-hydroperoxide isomer.

引用

页码：696 / 699

页数：4

共 18 条

[1]

Axelrod B., 1981, METHODS ENZYMOLOGY, P441, DOI DOI 10.1016/0076-6879(81)71055-3

[2] AEROBIC FORMATION OF KETO DIENE FROM LINOLEIC-ACID CATALYZED BY ONE OF THE 2 FORMS OF LIPOXYGENASE ISOLATED FROM BENGAL GRAM (CICERARIETINUM) [J].

BORTHAKUR, A ;

RAMADOSS, CS .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1986, 34 (06) :1016-1018

[3] PHYSICOCHEMICAL STUDIES ON 2 FORMS OF BENGAL GRAM LIPOXYGENASE - IMPLICATION FOR STRUCTURAL DIFFERENCES [J].

BORTHAKUR, A ;

RAMADOSS, CS ;

RAO, AGA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 958 (01) :40-51

[4]

COHEN JD, 1984, J CHROMATOGR, V313, P194

[5]

COOK HW, 1975, CAN J BIOCHEM CELL B, V53, P1220, DOI 10.1139/o75-167

[6] LIPOXYGENASE FROM POTATO TUBERS - PARTIAL PURIFICATION AND PROPERTIES OF AN ENZYME THAT SPECIFICALLY OXYGENATES 9-POSITION OF LINOLEIC ACID [J].

GALLIARD, T ;

PHILLIPS, DR .

BIOCHEMICAL JOURNAL, 1971, 124 (02) :431-&

[7] ANAEROBIC REACTION BETWEEN LIPOXYGENASE, LINOLEIC ACID AND ITS HYDROPEROXIDES [J].

GARSSEN, GJ ;

VLIEGENTHART, JF ;

BOLDINGH, J .

BIOCHEMICAL JOURNAL, 1971, 122 (03) :327-+

[8]

HAMBERG M, 1967, J BIOL CHEM, V242, P5329

[9] CHARACTERIZATION OF 2 ISOENZYMES OF LIPOXYGENASE FROM BUSH BEANS [J].

HURT, GB ;

AXELROD, B .

PLANT PHYSIOLOGY, 1977, 59 (04) :695-700

[10] 5-LIPOXYGENASE FROM POTATO-TUBERS - IMPROVED PURIFICATION AND PHYSICOCHEMICAL CHARACTERISTICS [J].

MULLIEZ, E ;

LEBLANC, JP ;

GIRERD, JJ ;

RIGAUD, M ;

CHOTTARD, JC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 916 (01) :13-23

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