APPLICATION OF THE PCR TECHNIQUE TO THE DETECTION OF HEPATITIS-A VIRUS IN THE ENVIRONMENT

被引：8

作者：

DIVIZIA, M ^{[1
]}

MORACE, G ^{[1
]}

GABRIELI, R ^{[1
]}

PISANI, G ^{[1
]}

PANA, A ^{[1
]}

机构：

[1] IST SUPER SANITA,VIROL LAB,I-00161 ROME,ITALY

来源：

WATER SCIENCE AND TECHNOLOGY | 1993年 / 27卷 / 3-4期

关键词：

HAV; ENVIRONMENTAL SAMPLES; WATER CONCENTRATION; PROBES; PCR;

D O I：

10.2166/wst.1993.0350

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Rapid, sensitive and specific techniques are needed to detect Hepatitis A virus in environmental samples. We have applied the method of Jansen et al. to concentrate river samples. In the analysis of 13 samples only two proved to be positive using the Elisa method (15.3%). Three samples were positive on cell culture (23%). The hybridization test resulted in 38.4% positive samples compared to the 67% obtained using the PCR method. The PCR showed the highest sensibility when compared to the traditional methods (cell culture and Elisa) and the differences were statistically significant. The difference between hybridization test and PCR was not statistically significant.

引用

页码：223 / 225

页数：3

共 8 条

[1] ISOLATION OF WILD-TYPE HEPATITIS-A VIRUS FROM THE ENVIRONMENT [J].