AN INVESTIGATION OF 3-FUNCTIONALIZED PYRROLE-MODIFIED GLUCOSE-OXIDASE FOR THE COVALENT ELECTROPOLYMERIZATION OF ENZYME FILMS

被引：34

作者：

YONHIN, BFY ^{[1
]}

LOWE, CR ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,INST BIOTECHNOL,TENNIS COURT RD,CAMBRIDGE CB4 1QT,ENGLAND

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1994年 / 374卷 / 1-2期

关键词：

D O I：

10.1016/0022-0728(94)03364-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glucose oxidase (GOx) was modified chemically with 3-carboxymethyl pyrrole by a carbodiimide coupling reaction. A maximum of 20 pyrrole moieties was attached to lysyl residues of the enzyme. They were shown to be electro-oxidizable. Copolymerization of the pyrrole-modified enzyme with pyrrole monomers resulted in polymer films exhibiting higher immobilized enzyme activity compared with previously studied copolymerized enzyme films using N-derivatized pyrrole. Optimization of the enzyme electrodes was performed with respect to the deposition potential, the amount of enzyme in the polymerization medium and the charge consumed for polymer deposition. The storage stability of the enzyme electrodes was determined.

引用

页码：167 / 172

页数：6

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