COMPUTER RECONSTRUCTION OF NUCLEOLAR ARCHITECTURE BY INTERACTIVE 3-DIMENSIONAL COLOR DISPLAY

被引:2
作者
GERAUD, G
SOYER, A
HERNANDEZVERDUN, D
机构
[1] UNIV PARIS 07,F-75252 PARIS 05,FRANCE
[2] UNIV PARIS 06,MINERAL CRISTALLOG LAB,F-75252 PARIS 05,FRANCE
来源
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE | 1991年 / 18卷 / 04期
关键词
3D RECONSTRUCTION; NUCLEOLUS; SERIAL SECTIONS;
D O I
10.1002/jemt.1060180404
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The first complete three dimensional ultrastructural reconstruction of pancreatic cell nucleoli, was done using EM and computer 3D-assisted reconstruction of serial sections with interactive 3D back-to-front and color display methods based on voxel representation. The purpose of the study was to depict the architecture of the nucleolar components. We obtained information about the location of the nucleolus within the nuclear volume and about the shape and polarity of the 3 main nucleolar territories.
引用
收藏
页码:354 / 359
页数:6
相关论文
共 29 条
[11]   NUCLEOLAR STRUCTURE [J].
GOESSENS, G .
INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1984, 87 :107-158
[12]  
GOESSENS G, 1979, BIOL CELLULAIRE, V35, P211
[13]  
HADJIOLOV AA, 1985, CELL BIOL MONOGR, V12, P130
[14]  
Hernandez-Verdun D, 1986, Methods Achiev Exp Pathol, V12, P26
[15]   3-DIMENSIONAL RECONSTRUCTIONS OF NUCLEOLUS-ORGANIZING REGIONS IN PHA-STIMULATED HUMAN-LYMPHOCYTES [J].
HOZAK, P ;
NOVAK, JT ;
SMETANA, K .
BIOLOGY OF THE CELL, 1989, 66 (03) :225-233
[16]  
JORDAN EG, 1981, J CELL SCI, V52, P373
[17]   STRUCTURE AND DEVELOPMENT OF NEURONAL CONNECTIONS IN ISOGENIC ORGANISMS - VARIATIONS AND SIMILARITIES IN OPTIC SYSTEM OF DAPHNIA-MAGNA [J].
MACAGNO, ER ;
LOPRESTI, V ;
LEVINTHAL, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (01) :57-61
[18]  
MAZIOTTA JC, 1977, COMPUT BIOL MED, V7, P265
[19]  
MEDINA FJ, 1981, BIOL CELL, V42, P79
[20]  
MEDINA FJ, 1983, BIOL CELL, V48, P31