INHIBITION OF SV40 GENE-EXPRESSION BY MICROINJECTED SMALL ANTISENSE RNA AND DNA-MOLECULES

被引：21

作者：

GRAESSMANN, M

MICHAELS, G

BERG, B

GRAESSMANN, A

机构：

[1] FREE UNIV BERLIN,INST MOLEK BIOL & BIOCHEM,ARNIMALLEE 22,BERLIN 33,GERMANY

[2] NIH,BETHESDA,MD 20892

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 01期

关键词：

D O I：

10.1093/nar/19.1.53

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We tested the impact of antisense RNA and DNA molecules on SV40 gene expression by microinjection into TC7 cells. Short antisense stretches, complementary to either hairpin or loop structures on the T antigen mRNA, inhibited T antigen synthesis. In contrast, full-length antisense RNA and DNA molecules did not effect T antigen synthesis.

引用

页码：53 / 59

页数：7

共 43 条

[11]

GRAESSMANN M, 1983, METHOD ENZYMOL, V101, P482

[12] MICROINJECTED SIMIAN VIRUS-40 CRNA IS SPLICED, AS EVIDENCED BY ELECTRON-MICROSCOPY [J].

GRAESSMANN, M ;

GRAESSMANN, A ;

WESTPHAL, H .

JOURNAL OF VIROLOGY, 1983, 48 (01) :296-299

[13] THE 2ND LARGE SIMIAN VIRUS-40 T-ANTIGEN EXON CONTAINS THE INFORMATION FOR MAXIMAL CELL-TRANSFORMATION [J].

GRAESSMANN, M ;

GUHL, E ;

BUMKEVOGT, C ;

GRAESSMANN, A .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 180 (01) :111-129

[14] THE ROLE OF ANTISENSE RNA IN GENE-REGULATION [J].

GREEN, PJ ;

PINES, O ;

INOUYE, M .

ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :569-597

[15] METALLOTHIONEIN [J].

HAMER, DH .

ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :913-951

[16] TRANSLATION OF MESSENGER-RNA INJECTED INTO XENOPUS OOCYTES IS SPECIFICALLY INHIBITED BY ANTISENSE RNA [J].

HARLAND, R ;

WEINTRAUB, H .

JOURNAL OF CELL BIOLOGY, 1985, 101 (03) :1094-1099

[17] SPECIFIC REGULATION OF GENE-EXPRESSION BY ANTISENSE, SENSE AND ANTIGENE NUCLEIC-ACIDS [J].

HELENE, C ;

TOULME, JJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1049 (02) :99-125

[18] FORMATION OF THE 3' END OF U1 SNRNA REQUIRES COMPATIBLE SNRNA PROMOTER ELEMENTS [J].

HERNANDEZ, N ;

WEINER, AM .

CELL, 1986, 47 (02) :249-258

[19] CONSTITUTIVE AND CONDITIONAL SUPPRESSION OF EXOGENOUS AND ENDOGENOUS GENES BY ANTI-SENSE RNA [J].

IZANT, JG ;

WEINTRAUB, H .

SCIENCE, 1985, 229 (4711) :345-352

[20] INHIBITION OF THYMIDINE KINASE GENE-EXPRESSION BY ANTI-SENSE RNA - A MOLECULAR APPROACH TO GENETIC-ANALYSIS [J].

IZANT, JG ;

WEINTRAUB, H .

CELL, 1984, 36 (04) :1007-1015

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