FORMATION OF HYDROGEN-PEROXIDE DURING PRECIPITATION OF RED-CELLS WITH PERCHLORIC-ACID - A CAUTIONARY NOTE FOR PRECISE DETERMINATION OF PYRUVATE, GSH, AND NAD(P)H

被引：26

作者：

GALLEMANN, D ^{[1
]}

EYER, P ^{[1
]}

机构：

[1] UNIV MUNICH,INST PHARMACOL & TOXIKOL,NUSSBAUMSTR 26,W-8000 MUNICH 2,GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 191卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90230-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The instability of pyruvate in neutralized perchloric acid extracts of erythrocytes is well-known from the literature. As shown in this study, the main reason for this phenomenon is a reaction of pyruvate with hydrogen peroxide, which is formed from oxyhemoglobin during acid precipitation. Thereby, pyruvate is oxidized to carbon dioxide and acetate. This decomposition is easily prevented by addition of catalase to the neutralized extracts, whereby authentic pyruvate can be determined precisely. Similarly, GSH and NAD(P)H oxidation in the extracts is prevented by addition of catalase. © 1990.

引用

页码：347 / 353

页数：7

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