ALTERATIONS IN MEMBRANE FLUIDITY OF DIABETIC POLYMORPHONUCLEAR LEUKOCYTES

被引：25

作者：

KANTAR, A ^{[1
]}

GIORGI, PL ^{[1
]}

CURATOLA, G ^{[1
]}

FIORINI, R ^{[1
]}

机构：

[1] UNIV ANCONA,DEPT BIOCHEM,I-60100 ANCONA,ITALY

来源：

BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY | 1991年 / 46卷 / 03期

关键词：

D O I：

10.1016/0885-4505(91)90090-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasma membrane fluidity of polymorphonuclear leukocytes was investigated in 28 patients with insulin dependent diabetes mellitus and 30 healthy controls. Membrane fluidity was measured by steady-state fluorescence anisotropy of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated into the plasma membrane. The fluorescence anisotropy values in resting (unstimulated) polymorphonuclear leukocytes from diabetic subjects were significantly higher than those of controls (0.318 ± 0.003 vs 0.287 ± 0.003, P < 0.001). The addition of the respiratory burst stimulus phorbol myristate acetate induced a stable increase in fluorescence anisotropy values in both groups. Fluorescence anisotropy values of stimulated polymorphonuclear leukocytes from the diabetic and control groups were not significantly different (P > 0.05). These data demonstrate a decrease in plasma membrane fluidity of resting polymorphonuclear leukocytes obtained from diabetic subjects. This finding could be in part explained by an increase in their basal respiratory burst activity. © 1991.

引用

页码：422 / 426

页数：5

共 27 条

[21] LIPID STRUCTURAL ORDER PARAMETERS (RECIPROCAL OF FLUIDITY) IN BIOMEMBRANES DERIVED FROM STEADY-STATE FLUORESCENCE POLARIZATION MEASUREMENTS [J].