2-DIMENSIONAL MAPPING BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PYRIDYLAMINO OLIGOSACCHARIDES FROM VARIOUS GLYCOSPHINGOLIPIDS

被引：28

作者：

OHARA, K

SANO, M

KONDO, A

KATO, I

机构：

[1] Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Otsu, Shiga, 520-21

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 586卷 / 01期

关键词：

D O I：

10.1016/0021-9673(91)80022-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method to map sugars two-dimensionally for the analysis of the structures of oligosaccharides from glycosphingolipids is described. Nine neutral and ten acidic oligosaccharides were obtained from glycosphingolipids by endoglycoceramidase digestion and labelled with 2-aminopyridine. The pyridylamino oligosaccharides were clearly separated by high-performance liquid chromatography on commercially available C18-silica and amide-silica column. All compounds tested were mapped without any overlapping. The separation of the pyridylamino oligosaccharides on the C18-silica column depended on the numbers and positions of sialic acid and N-acetylhexosamine residues; on the amide-silica column, the separation depended on the total number of sugar residues.

引用

页码：35 / 41

页数：7

共 17 条

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