MEASUREMENT AND COMPUTATION OF MURINE INTERLEUKIN-4 AND INTERFERON-GAMMA BY EXPLOITING THE UNIQUE ABILITIES OF THESE LYMPHOKINES TO INDUCE THE SECRETION OF IGG1 AND IGG2A

被引：9

作者：

AGREWALA, JN ^{[1
]}

RAGHAVA, GPS ^{[1
]}

MISHRA, GC ^{[1
]}

机构：

[1] INST MICROBIAL TECHNOL, PB 1304, SECTOR 39A, CHANDIGARH 160014, INDIA

来源：

JOURNAL OF IMMUNOASSAY | 1993年 / 14卷 / 1-2期

关键词：

INTERLEUKIN-4; INTERFERON-GAMMA; COMPUTER PROGRAM; IMMUNOGLOBULIN ISOTYPE;

D O I：

10.1080/15321819308019842

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific and new method for measuring Interleukin-4 and Interferon-gamma, based on the estimation of IgG1 and IgG2a isotypes secretion from B cells is described. An antagonizing effect of Interferon-gamma in the production of IgG1 induced by Interleukin-4 was neutralized by using antibody to Interferon-gamma. Similarly, the interference of Interleukin-4 in the Interferon-gamma mediated enhancement of IgG2a production was blocked by anti-Interleukin-4 antibody. The high concentrations of Interleukin-4 and Interferon gamma inhibited the secretion of IgG1 and IgG2a respectively. Therefore, in the assay described, the samples containing the cytokines were so diluted that their activity fell into the non-inhibitory zone. A computer program has also been developed for determining the concentrations of lymphokines.

引用

页码：83 / 97

页数：15

共 22 条

[1] A BIOLOGIC ASSAY FOR IL-4 - RAPID FLUORESCENCE ASSAY FOR IL-4 DETECTION IN SUPERNATANTS AND SERUM [J].

CUSTER, MC ;

LOTZE, MT .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 128 (01) :109-117

[2]

DELESPESSE G, 1989, J IMMUNOL, V142, P134

[3] B-CELL-STIMULATORY FACTOR-I (BSF-1) PROMOTES GROWTH OF HELPER T-CELL LINES [J].

FERNANDEZBOTRAN, R ;

KRAMMER, PH ;

DIAMANTSTEIN, T ;

UHR, JW ;

VITETTA, ES .

JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 164 (02) :580-593

[4]

FINKELMAN FD, 1988, J IMMUNOL, V141, P2335

[5] LYMPHOKINE CONTROL OF INVIVO IMMUNOGLOBULIN ISOTYPE SELECTION [J].

FINKELMAN, FD ;

HOLMES, J ;

KATONA, IM ;

URBAN, JF ;

BECKMANN, MP ;

PARK, LS ;

SCHOOLEY, KA ;

COFFMAN, RL ;

MOSMANN, TR ;

PAUL, WE .

ANNUAL REVIEW OF IMMUNOLOGY, 1990, 8 :303-333

[6] B-CELL-STIMULATORY FACTOR-II (BETA-2 INTERFERON) FUNCTIONS AS A 2ND SIGNAL FOR INTERLEUKIN-2 PRODUCTION BY MATURE MURINE T-CELLS [J].

GARMAN, RD ;

JACOBS, KA ;

CLARK, SC ;

RAULET, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7629-7633

[7] RECIPROCAL EXPRESSION OF INTERFERON-GAMMA OR INTERLEUKIN-4 DURING THE RESOLUTION OR PROGRESSION OF MURINE LEISHMANIASIS - EVIDENCE FOR EXPANSION OF DISTINCT HELPER T-CELL SUBSETS [J].

HEINZEL, FP ;

SADICK, MD ;

HOLADAY, BJ ;

COFFMAN, RL ;

LOCKSLEY, RM .

JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (01) :59-72

[8] B-CELL STIMULATORY FACTOR-I (INTERLEUKIN-4) IS A POTENT CO-STIMULANT FOR NORMAL RESTING LYMPHOCYTES-T [J].

HULI, J ;

SHEVACH, EM ;

MIZUGUCHI, J ;

OHARA, J ;

MOSMANN, T ;

PAUL, WE .

JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 165 (01) :157-172

[9]

LAYTON JE, 1985, J MOL CELL IMMUNOL, V2, P155

[10]

MOND JJ, 1986, J IMMUNOL, V137, P3534

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