PRESYNAPTIC MODULATION OF AMINO-ACID RELEASE FROM SYNAPTOSOMES

被引：16

作者：

SHERMAN, AD

HEGWOOD, TS

BARUAH, S

WAZIRI, R

机构：

[1] Department of Psychiatry, College of Medicine, University of Iowa, Iowa City, 52242, Iowa

来源：

NEUROCHEMICAL RESEARCH | 1992年 / 17卷 / 02期

关键词：

GLUTAMATE; GABA; NMDA; SYNAPTOSOMES;

D O I：

10.1007/BF00966789

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using synaptosomes prepared from whole rat brain, the spontaneous, calcium-independent, and calcium-dependent release of glutamate and GABA was assessed. Time intervals of 1-30 seconds were studied. Spontaneous release of glutamate (but not GABA) was elevated by 10-mu-M NMDA or AMPA by thirty seconds. This stimulation was partially calcium-dependent. Calcium-dependent release induced by 30 mM KCl was biphasic, confirming previous findings. This release was stimulated at all time periods by the presence of 10-mu-M NMDA or AMPA in an antagonist-sensitive manner. These data suggest that glutamate and GABA are released from vesicular stores in rat synaptosomes and that some of this release is modulated by presynaptic glutamate receptors.

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页码：125 / 128

页数：4

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