PROTEIN DEAMIDASE FROM GERMINATING WHEAT GRAINS

被引：12

作者：

VAINTRAUB, IA

KOTOVA, LV

SHAHA, R

机构：

[1] Laboratory of Protein Chemistry, Moldavian University

来源：

FEBS LETTERS | 1992年 / 302卷 / 02期

关键词：

PROTEIN DEAMIDASE; GERMINATING SEED; WHEAT;

D O I：

10.1016/0014-5793(92)80432-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new enzyme catalyzing the deamidation of seed storage proteins was found in germinating wheat grains and was partially purified. It also acts on egg lysozyme, horse hemoglobin and reduced RNAse, glutamine and Gly-L-Gln-L-Tyr. No activity was observed when using ovalbumin, serum albumin, RNAse, insulin, asparagine and an asparagine-containing peptide. Only glutaminyl residues appear to be deamidated by this enzyme. It differs from transglutaminase and proved to be a true protein deamidase.

引用

页码：169 / 171

页数：3

共 17 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] IMMUNOCHEMICAL STUDIES ON ARACHIS HYPOGAEA PROTEINS WITH PARTICULAR REFERENCE TO RESERVE PROTEINS .2. PROTEIN MODIFICATION DURING GERMINATION [J].