DOMAINS OF THE HUMAN ANDROGEN RECEPTOR AND GLUCOCORTICOID RECEPTOR INVOLVED IN BINDING TO THE NUCLEAR MATRIX

被引:84
作者
VANSTEENSEL, B
JENSTER, G
DAMM, K
BRINKMANN, AO
VANDRIEL, R
机构
[1] UNIV AMSTERDAM,EC SLATER INST,1018 TV AMSTERDAM,NETHERLANDS
[2] ERASMUS UNIV ROTTERDAM,DEPT ENDOCRINOL & REPROD,3000 DR ROTTERDAM,NETHERLANDS
[3] MAX PLANCK INST PSYCHIAT,W-8000 MUNICH,GERMANY
关键词
STEROID RECEPTORS; C-TERMINAL DOMAIN; DNA BINDING DOMAIN; TRANSCRIPTION; NUCLEAR LOCALIZATION; NUCLEAR MATRIX; NUCLEAR MATRIX ACCEPTORS; INTERACTION; DISULFIDE BRIDGES;
D O I
10.1002/jcb.240570312
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Steroid receptors have been reported to bind to the nuclear matrix. The nuclear matrix is operationally defined as the residual nuclear structure that remains after extraction of most of the chromatin and all soluble and loosely bound components. To obtain insight in the molecular mechanism of the interaction of steroid receptors with the nuclear matrix, we studied the binding of several deletion mutants of the human androgen receptor (hAR) and the human glucocorticoid receptor (hGR) to the nuclear matrix. Receptor binding was tested for two different nuclear matrix preparations: complete matrices, in which most matrix proteins are retained during the isolation procedure, and depleted matrices, which consist of on ly a subset of these protei ns. The results show that the C-terminal domain of the hAR binds tightly to both depleted and complete matrices. In addition, at least one other domain of the hAR binds to complete matrices but not to depleted matrices. In contrast to the hAR, the hGR binds only to complete matrices. For this interaction both the DNA-binding domain and the C-terminal domain of the hGR are required, whereas the N-terminal domain is not. We conclude that specific protein domains of the hAR and the hGR are involved in binding to the nuclear matrix. In addition, our results indicate that the hAR and the hGR are attached to the nuclear matrix through different molecular interactions. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:465 / 478
页数:14
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