CONSTRUCTION AND CHARACTERIZATION OF A MULTILAYER ENZYME ELECTRODE - COVALENT BINDING OF QUINOPROTEIN GLUCOSE-DEHYDROGENASE ONTO GOLD ELECTRODES

被引:44
作者
JIN, W
BIER, F
WOLLENBERGER, U
SCHELLER, F
机构
[1] Max-Delbrück-Center of Molecular Medicine, D-13125 Berlin
[2] University of Potsdam, Max-Delbrück-Center of Molecular Medicine, D-13125 Berlin
关键词
D O I
10.1016/0956-5663(95)99221-6
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Multilayers of quinoprotein glucose dehydrogenase were assembled onto modified gold electrodes. As a primary modifier the bifunctional 3,3'-dithiodipropionic acid bis(N-hydroxysuccinimide ester) was chemisorbed. Glucose dehydrogenase was covalently bound to this activated electrode in a stepwise procedure. In the presence of glucose, the electrode functions as a sensor for electron accepters. Catalytic current, as observed for p-aminophenol, was used to characterize electrode performance. The dependence of the electrode response on the number of enzyme layers showed that the transition from a kinetic to a diffusion-limited sensor is reached at 6-7 enzyme layers. The response of multilayer electrode is stable over a broad range of pH and ionic strength of the bulk solution. It also shows good stability: after 2 months, 75% of its original activity remained.
引用
收藏
页码:823 / 829
页数:7
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